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The membrane was incubated with the primary antibody consisted of Bcl-2 antibody (1:1000, ABclonal, A0208), Bax antibody (1:30000, HUABIO, ET1603-34), Caspase-3 antibody (1:1000, ABclonal, A25309) and GAPDH antibody (1:10000, ABclonal, AC001) overnight